Official websites use. Share sensitive information only on official, secure websites. Sickle cell anemia is a blood disorder, known to affect the microcirculation and is characterized by painful vaso-occlusive crises in deep tissues.
During the last three decades, many scenarios based on the enhanced adhesive properties of the membrane of sickle red blood cells have been proposed, all related to a final decrease in vessels lumen by cells accumulation on the vascular walls.
Up to now, none of these scenarios considered the possible role played by the geometry of the flow on deposition. The question of the exact locations of occlusive events at the microcirculatory scale remains open. Here, using microfluidic devices where both geometry and oxygen levels can be controlled, we show that the flow of a suspension of sickle red blood cells around an acute corner of a triangular pillar or of a bifurcation, leads to the enhanced deposition and aggregation of cells.
Thanks to our devices, we follow the growth of these aggregates in time and show that their length does not depend on oxygenation levels; instead, we find that their morphology changes dramatically to filamentous structures when using autologous plasma as a suspending fluid. We finally discuss the possible role played by such aggregates in vaso-occlusive events. Sickle cell anemia SCA is an inherited hemoglobinopathy 1 affecting millions of people worldwide 2. It is caused by a point mutation resulting in the production of abnormal hemoglobin called hemoglobin S HbS 3.
When deoxygenated, HbS molecules polymerize into long fibers 4 , which can span the size of the red blood cell RBC , distorting it into the classical crescent shape at rest 5,6.
