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Pachiadakis, V. Karavassilis, A. Vasdeki, A. Melidou, A. Goudouva, P. Lisgara, E. Petinaki, A. Maniatis Larissa, GR. Objective: To evaluate a commercial kit for the detection of Salmonella spp.
Methods: A total of faecal specimens obtained consecutively from individual patients of Thessalia Central Greece were included in this study. Genomic DNA extractions were performed by use of commercially available isolation kits, while the commercial kit described above, according to the instructions of the manufacturer did real-time PCR.
Results obtained from Real-Time PCR assay were compared with bacterial culture results to determine sensitivity and specificity. Salmonella was detected by bacterial cultures in all, except two, the positive specimens. Repetition of two negative cultures after enrichment detected the pathogenic micro organisms. The mean time for the detection of Salmonella by Real-Time PCR was approximately 5β6 hours, while detection by traditional culture methods required 2β3 days.
Conclusion: The high sensitivity and specificity of this Real-Time PCR assay emphasizes the need of its application in a routine clinical laboratory for the rapid and accurate detection of Salmonella in faecal specimens. Savin, S. Huck, C. Rolland, M. Benderdouche, O. Faure, G. Noacco, J. Menotti, E. Candolfi, H. Pelloux, R.
