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Official websites use. Share sensitive information only on official, secure websites. The idea of collecting blood on a paper card and subsequently using the dried blood spots DBS for diagnostic purposes originated a century ago.
Since then, DBS testing for decades has remained predominantly focused on the diagnosis of infectious diseases especially in resource-limited settings or the systematic screening of newborns for inherited metabolic disorders and only recently have a variety of new and innovative DBS applications begun to emerge. For many years, pre-analytical variables were only inappropriately considered in the field of DBS testing and even today, with the exception of newborn screening, the entire pre-analytical phase, which comprises the preparation and processing of DBS for their final analysis has not been standardized.
Given this background, a comprehensive step-by-step protocol, which covers al the essential phases, is proposed, i. In a second step, the protocol was utilized during a pilot study, which was conducted on active drug users in the German cities of Berlin and Essen.
Keywords: Molecular Biology, Issue 97, Dried blood spots, filter paper cards, specimen storage, infectious diseases, hepatitis B virus, hepatitis C virus, human immunodeficiency virus. Download video stream. The idea of using blood collected on a paper card made of cellulose is ascribed to Ivar Christian Bang β , the father of modern clinical microanalysis 1, 2.
In , Bang determined glucose from eluates of dried blood spots DBS 3 and, later, also performed nitrogen measurements using the Kjeldahl method with this filter paper technique 2. Subsequently, several investigators reported on the use of DBS for serological testing to diagnose syphilis 2.